Introduction

New opportunities has been opened in the biomedicine by the use of next generation DNA sequencing techniques. In biomedicine this technique was selected by Nature methods in 2007. Before the development of this technique, Sanger enzymatic dideoxy technique was used that was developed in 1977. Sanger method was used for sequencing of a genome region by automated fluorescent project.

The development of techniques that allows the higher sequencing is came into begin after the decision of the determination of whole human genome sequence by International Community. These high-throughput sequencing (HTS), also called Next Generation Sequencing (NGS) has a platforms that are based on cyclic array sequencing implementations. The Sequencing of dense array of DNA features by iterative cycles of enzymatic manipulation and imaging-based data collection define the concept of cyclic-array sequencing. There are many commercial product which are based on this sequencing technology such as Roche’s 454, Illumina’s Genome Analyzer, ABI’s SOLiD and the Heliscope from Helicos. All these platforms give slightly different sequencing as well as the generation of array from each other but their flow of work is very similar according to concept. The sequencing of millions of short sequences is allowed by all of these products by which they are capable of producing a sequence of full human genome per week with the cost 200 fold that is very less compared to other previous methods (Ansorge, 2009; Magi et al, 2010).