Authors
Poonam Moon, Gunjan Jindal , Dr. Ranjeet Kumar Singh
Abstract
In recent days, there is constantly increasing or developing the techniques for human identification. From degraded human remains, DNA typing is a big task or challenge for forensic DNA experts not only in prospective of purification of DNA but also in data manipulation and interpretation of establishing the profiles of DNA, specifically in mass fatalities. Forensic anthropologists processed the human remains which are used for the purpose of genetic analysis. In future analysis, DNA’s condition (deoxyribonucleic acid) which is found in human remains, may become an issue. Scanning electron microscope (SEM) identified the bone’s damage which is indicates differences that may exists in quantity and quality of DNA extracted. DNA profiling has been developed the core of human identification with new era of revolution of DNA, molecular biology and PCR techniques. In this paper, discussed about the various methods for DNA extraction from human remains samples. Keywords: DNA Extraction, PCR Amplification, Human Remains, Scanning Electron Microscope (SEM)
Introduction
In ancient time, DNA has suffered from so many problems as it is preserved in numerous states of degradation and small amounts (Rohland and Hofreiter, 2007). Forensic anthropologist, forensic pathologist and forensic geneticists are faced a challenge with the human remains which are found in buried, decomposed and skeletonized condition. They use various investigative techniques due to which they are able to establish the potential identification in which includes the details of skeletal by their relative and special features includes scar or tattoo mark, fingerprint impression and dental profile. These techniques are required for the purpose of comparison between informative and huge ante-mortem (AM) and post-mortem (PM) data of human remains but in cases of missing person and mass casualty, the information of ante-mortem is not valuable or less informative. In some cases, such conventional methods have to be used but they cannot provide the positive result then used the DNA typing method which provide positive identification of human. It is very rapid test and analyzed or performed by automatic machines (Arismendi, Baker and Matteson, 2004; Siriboonpiputtana, 2018). In some cases, human remain samples includes many types of sample such as bone, blood, nail, hair and tissue etc., considered as a biological evidence which are used for the purpose of analysis and identification of cadaver victims.
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| APA Style | Moon, P. Jindal, G. and Singh, R (2019). DNA Extraction from Human Remains through Different Methods. Academic Journal of Anthropological Studies, 2(2), 16-20. |
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